LOCUS       40924_45693        8887 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUH-EX DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Onizuka T, Akiyama H, Umeda Y, Hirano A, Ikeuchi M.
  TITLE     Expression vector pUH-EX for cyanobacteria
  JOURNAL   Published Only in Database (2002)
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Onizuka T, Akiyama H, Umeda Y, Hirano A, Ishibashi M, Ikeuchi M, 
            Inoue Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2002) Takuo Onizuka, Toray Reserch Center, Inc., 
            Biological Sciences Department; Tebiro 1111, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9965, Fax:81-467-32-0414)
REFERENCE   3  (bases 1 to 8887)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2002)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2002) Takuo Onizuka, Toray Reserch Center, Inc., Biological 
            Sciences Department"; volume: " Tebiro 1111, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9965, Fax"; pages: "81-467-32-0414"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(147..175)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    250..255
                     /label=XbaI cloning site
                     /note="XbaI cloning site"
     misc_feature    256..261
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    261..266
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    271..276
                     /label=SacI cloning site
                     /note="SacI cloning site"
     misc_feature    277..282
                     /label=EcoRI cloning site
                     /note="EcoRI cloning site"
     primer_bind     complement(292..308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(316..332)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(340..370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(385..406)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(694..1282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1456..2313)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2314..2418)
                     /label=AmpR promoter
     primer_bind     2892..2908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6756..8557
                     /note="Promoter of the phycocyanin gene of the
                     cyanobacterium Synechococcus sp. PCC7942"
                     /regulatory_class="promoter"
     misc_feature    8556..8561
                     /label=NcoI cloning site
                     /note="NcoI cloning site"
     misc_feature    8882..8887
                     /label=EcoRV cloning site
                     /note="EcoRV cloning site"
ORIGIN
        1 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
       61 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
      121 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
      181 agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt
      241 gcactgaaat ctagaggatc cccgggtacc gagctcgaat tcgtaatcat ggtcatagct
      301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      421 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2461 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     2521 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     2581 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2641 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     2701 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     2761 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     2821 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     2881 gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcaaggaga tggcgcccaa
     2941 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     3001 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     3061 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct gcaagtcaac
     3121 agccgcgatg agttcaaccc ttggtcgctg gaggttgcga tcgacgcacg gctgagcggc
     3181 agctactact acctagttgc acgcggcaac ctacctctgg agctgggacg cctgacgccc
     3241 gcgcctcagc taactagcga ggtgcagttc gagacgggct tttaccgcgc caaggctgag
     3301 cacagcttcg gctgctgcgt agtcgatcac cgtccgatcg ttcggatctc gacagcgtcc
     3361 ggcgtctaag tctcatcaca gccaggggcg atcgcgctct ggcttgccga tcaattcgag
     3421 gcagccttgc cacctgccgt attcattcca gctatagtta tcggcaatca aagcaaaacc
     3481 ccccccaggc caggtcggca aactttgccc aggggaggtc aggaaaaata actcaacaga
     3541 agtatggcat ttatcgccaa tcatctgcaa aggtcgcaga tcccgacccg ctgcgaatta
     3601 attgatcaac ttgaccggct gcccccaaac tggcgcttct gcgtcgttaa cagtcgcaag
     3661 gcaccatttg accctggctg gcaaaataac ccgcttgacc atgctgctgt tgctgaacgt
     3721 atccgcagcg atcggcgcgt taccggaatc ggtctgctta ctggccccgc aagcggtggg
     3781 ttaatcgcag ttgacttcga tggcccaacg gctcatgagg cactgccaga agggctgacg
     3841 ctgacagaat taccgcccac agttgcatat acatctggca agccaggacg ccaccaacgg
     3901 ctctatcagg taccccaaga gcgatgggct gcgatcgcca cgcagaagct tcacagccca
     3961 gacggtgatc tgttagagct gcgttggaac aagctgcaat cagtcatcgt tggtcaacac
     4021 cctgagaccg gcgcatatcg ctgggtggaa ggctgcgcac cttgggagat cgaggttgca
     4081 gaggccccgc ccgaattgct ggatgcaatg gagcggcaag accggcagac cgcgccgaaa
     4141 tcctataagc cgatcgtcag tgctccagct tctgatgatg aggtcgcgat cgcccgcacc
     4201 atgctggccc atgtacctgc cagctacgca gacgactacg agagctgggt ggctgtcggg
     4261 atggccctgc agtcagtcag cgatgccctg ttagatgact ggattgcatg gtcagcgcaa
     4321 tcgtccaagt tcgacggcaa ccgcaagcta gagcggaagt gggccagctt caagggcagc
     4381 ggcatcacga tcgccacact ggccaagctg gccaaagaag gcggctaccg cccgccaaag
     4441 ctgactcgac aagagcagcg ggaatatcag cgcgaggtcg cgaacgagtg gcagcggctg
     4501 acccgtagcc ctaaccgctt acacaccggc gggtacatca acggcgacta catcccgcag
     4561 ccggaacagc atcgccttgt ctgcctgaat gctgcgatgg ggacgggcaa gacagaggcg
     4621 atcgcggggc atctccagcc gctcattggt accggcattc ctgtcgtgtt gatcacgcat
     4681 cgccgtacct tgggcgcggc cttgggcaag cgcttagggg tgccgtgggc tgaagaatgc
     4741 gttcccggtt ccgaccttcg gatgatcggc cttggcggtt gcctagactc catgcacccc
     4801 gccagcaagc tgcgcttcag cgcccacgga tggcaacggg cgatcgtcgt catcgatgag
     4861 gtggaactgt gggctgagca cctgctgagc ggcaagaccg agatcgctaa acatcgcccc
     4921 gaagtcatgg ctgaaattgc tggactactc gcaggggggc ggcaggttat cgccgctgac
     4981 gcgatgctgt cggatgtgtc gatcgccctg ttggaagcac tgacaggatc aaccgcatat
     5041 ctggtcagca gtaccgtcca acccttcagc ggtgctccgg tctatcaggt tggcagcgat
     5101 cgcgaactca tggcgatggc ggtcgaaatg gttcaggcgg gcaagcgctt agcagtcttc
     5161 tgcgaccgcc agaagctgag ccagtcgcgg ccgtccctgt attccgcagc gacgatcgcc
     5221 caacagctct ccgaagcctg cggtcagccg gttccggtca tcgacagcga agccaataag
     5281 cggggggctt tggaaacgct gaagctcaac ccgcagatgt tcatcaactg ttgcccccac
     5341 cttgtcttca cgagcgtgat cgatgctggc ttgtcgatca cccaaggggt aggcgatcgc
     5401 ccatttgatg ccgtgctgat cttcgccgga gctgggcaca tcgccccagg tgcgatcgcg
     5461 cagattgcag gacgggtgcg gagcaacgtc ccccgattca tcacctgccc cgacacgtcc
     5521 aatttgatgc gagctggcaa cggtagcatc gacccaaccg aggttgccga cggcctgaag
     5581 cggcaccaac aagtgatctt ggctcagctg tcacaggcgg cgatcaagat cggcgagccg
     5641 gtcgcatcgc aagcctactt gactgcatgg gcagaattgg ccgcccgtcg caaccgtgac
     5701 gggttgacct atgccgccac ggtggagcag cttctacggc tcaacgggtt cacttggcaa
     5761 gactgggacg gcgcgatcga tccctttctt caagaggtcg ttgacgaact gcaacgggag
     5821 actgctgaag cgctggctga tgcctcagcc gaggcgatcc tgagttcacc tgatctgacc
     5881 ccgaaggaag ctgaggcgat cgcgtctgcg gacttcgcta acgcggaaca gttggctgcc
     5941 cttgctcggc accgctgcca gcaacgctgg ggagactgca cccgtgaact actagccgct
     6001 gatgacgacg gtctatacgc cccgctaaga ctgctgttct ggctcagcct tggcaaggat
     6061 gccgtgccgc tcgcgatgcc gccaagctcg acaaacggcg cgatcgcccg atgttcggac
     6121 ccgatcttgt gggcagctgt atcgcgcccc aagtgcggta cctgcatggg ctgaaggtgg
     6181 aagagttgct gcaacgccgc gactggttca gcgttgatga tccgctgatt caagagatcg
     6241 tcacgaccgc cacaagccac cgggctagtg ccgcacagat cctcaagatt cagatcaccg
     6301 acaagaccag agccagcacc atcgtgcggg cgctgctgcg gatgatcggc ttcaagctca
     6361 aggcacggca gaagcgcatc gcgggcaagt ccacttggat ctaccaagtc gtgccgaatg
     6421 aggcgattgc tgaactgccg cttgaggcga tcgaagctca ttggcttgaa aacgcccgat
     6481 ctagtcaggg actggggagc tgtcacgaaa tccctaatag aagatcaaaa ccaatcactg
     6541 cgcattttcg tgacaccgcg tcaggtcagg tcagcactac atctacggag accgcagcat
     6601 gacacaagcc gaactcgatc agctttggac aactgcgatc gccgcgatcg agaacgaagg
     6661 cttgcgatgg tttgcggcta accgtgccgc gcctacagct tcgatagtgg aacgctggcc
     6721 atcgcagcac ccaagcacat gtggccaatg ctcgagggta gggaatgcca gcaacggtga
     6781 tcgggccatt gctctcaatc tcgaggcgat cgccctgaaa gcgttgatcc cctagctgcc
     6841 aaaccaactg cggttgtgtc ttgcgtgtct cgctgcgtag cagagcagac gcggctcctt
     6901 cactgcgcaa ccgttgcagc aaactcggta gggcggcatc aggtaggtct ggggtcggtt
     6961 ttaaccagac ttcccagccc tgcggttggg cgatttcgac gtcaaacccc ttggcccgcc
     7021 attgcgcggc gctggcctca gcactctcaa agctgcggtg ctgactaatg actaattgcg
     7081 atcgctgctc gggggtcggt agggggacag gggcaatcgc caccgtcagc cgttgggccc
     7141 gaaactgacg cggttgacca ttgaccgtga atcggagcgt cagcgatcgc tgggcaccag
     7201 ccacaatttg caggcgatcg ctcggctgcc gaccaaagcg ctggacaatt cccactcgta
     7261 atgttgggtt gagaggtgct tgggcttggg tcaccggtgc cgacggaaaa agtcccagcc
     7321 cgatcgctag gggaagagcc caaagtttca gcatggcttt agagacagca gcaaatgctc
     7381 gagcagagct ttttaagtgt gacaggccgc tggagcgatc cgttccttcg accgctagac
     7441 tgccctcagg gctaaaaatc cttgtcttaa gtctctgtct ttgttgtgct ccaggatgag
     7501 atgctcaccc taatctcgac cgctctgact tggggtctgc ggctgttcgg ttgcttttgg
     7561 ctcatgggcg ggctgctggc actacagcaa gcgcggcaag cccatctaat ggacaacttg
     7621 ctcgaagccc tcagccaaga aaaggaagat cgcctcacca gtcggtttct gctcattggc
     7681 tccgtgctga ccttcatgag tggcgctggc ttgatcctta gcagccagtg ggtcttaatc
     7741 ccgctagcct tgctcgtcct gtctcagctc atttactttc gcctcaagga acagcgcttt
     7801 cagcgggcaa ccaacgagga agagcgcctc gatgccacag ttcaatcctc gactgaaaac
     7861 gcctttatcg tctccttggt cgttgcgatc gctgcttttt tgtgctggcg cttgggcgga
     7921 ctgcgttaag gctacagcct gggttcctca tgactctcag tctcagctgc atgctggttg
     7981 cttgccctgc tgccggtccc atgagccagc gatcgctcag ttcaagaatg taaatgaata
     8041 ttacaaacct tcagctcgta acgatttcag atccaaaaga tgtagctgtt gggtcagcca
     8101 taagtttttc ttcaacttct caagcgatcg tcgaccatca acttaaagca tctttacaaa
     8161 gctagagatt gaccctagtc gtggacgact tacagattca ggattgatac ggccaatccc
     8221 gatcgcgatc gctctaaatc cccgtcagtc agagcttcac aatttttagc gaatcttgtg
     8281 gccgcgatcg ttgtataaga atgccaaggc aactggataa ggttcactaa tcgttgctaa
     8341 gcgacagtga actgcgccaa ttgcctgaca ggcccctctc gtttaacaaa cgatttaatg
     8401 taaatcattg ttaagagtct ctcacaatcg agagttttct tgaagaatga tggggacggt
     8461 tcaggtgcag ggtttccctg ctagagaatg cgaaaaaacc gcgttctcgt tttaggaatc
     8521 gagagtcaat aaaagtcgag aacaggagac tggttccatg gctgaaagcc agtccctgcc
     8581 cgagtttgct cgcgctaatg gcgttgcgcc tcaggcgata catcaggcga tcgcagccgg
     8641 tcggatcact tcagtctgga aggtcggcag tcggtggcac gtcgatccgg ttgctgcggc
     8701 tcgcgagtgg gcagcaaaca ccgatccgag ccggatcagg aacgatggtg gcgggcgtgg
     8761 caagcgtcgt gagcctccat cagccgagca actagaggcg cggcgtctga aagcgcacta
     8821 ccgcgccgag ctgctccggc tcgacgttga agaacgcgag cgatcgctag ttgatgctga
     8881 ggatatc
//
